제품 상세
Aspergillus flavus 및 A. parasiticus를 검출하기 위한 선택 확인 배지
AFPA (Aspergillus Medium)
Code: CM0731
Typical Formula* | gm/litre |
Peptone | 10.0 |
Yeast Extract | 20.0 |
Ferric ammonium Citrate | 0.5 |
Dichloran | 0.002 |
Agar | 15.0 |
pH 6.3 ± 0.2 @ 25°C |
|
* Adjusted as required to meet performance standards
CHLORAMPHENICOL SUPPLEMENT
Code: SR0078
Vial contents |
SR0078E (1 vial per 500ml medium) |
SR0078H (1 vial per 2 litres medium) |
per litre |
Chloramphenicol |
50mg |
200mg |
100mg |
Directions
Suspend 22.75 g in 500ml (45.5 g/l) of distilled water and heat to dissolve completely. Reconstitute one vial SR0078E per 500ml medium or one vial SR0078H per 2 litres medium, as directed. Add the vial contents to the AFPA Base. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C. Mix well and pour into sterile Petri dishes.
Appearance
Dehydrated medium: Straw coloured, free-flowing powder
Prepared medium: Straw coloured gel
Quality control
Positive controls: | Expected results |
Aspergillus flavus ATCC® 22547 | White mycelium, buff spores, orange underside |
Aspergillus parasiticus ATCC® 28285 | White mycelium, cream spores, orange underside |
Negative controls: |
|
Aspergillus brasiliensis ATCC® 16404 * | White mycelium, black spores, yellow underside |
Escherichia coli ATCC 25922 * | No growth |
* This organism is available as a Culti-Loop®