제품 이미지
Amies Transport Medium 제품소개 : 미생물배양용 분말배지  브 랜 드  : Oxoid

제품 상세

병원성 균의 생존력을 연장시키기 위해 숯(charcoal)이 포함된 수송배지


Typical Formula*

gm/litre

Charcoal pharmaceutical

10.0

Sodium chloride

3.0

Sodium hydrogen phosphate

1.15

Potassium dihydrogen phosphate

0.2

Potassium chloride

0.2

Sodium thioglycollate

1.0

Calcium chloride

0.1

Magnesium chloride

0.1

Agar

4.0

pH 7.2 + 0.2 @ 25°C


* Adjusted as required to meet performance standards 


Directions

Suspend 20 g in 1 litre of distilled water. Bring to the boil to dissolve the agar completely. Distribute into small, screwcap bottles, stirring meanwhile to keep the charcoal evenly suspended. Screw down the caps firmly on the completely filled bottles. Sterilise by autoclaving at 121°C for 15 minutes. Invert the bottles whilst cooling to distribute the charcoal uniformly. Store in a cool place.


Appearance
Dehydrated medium: Black coloured, free-flowing powder
Prepared medium: Straw coloured semi-solid gel


Quality control

Positive controls:

Expected result at 35°C

Staphylococcus aureus ATCC® 25923 *     

Good growth

Escherichia coli ATCC® 25922 *

Good growth

Negative control:

 

Uninoculated medium

No change


* This organism is available as a Culti-Loop®


Precautions

It is important that the charcoal is properly suspended in the medium, invert the bottles when the bottles are cool but the agar still liquid.
During preparation of the medium, avoid prolonged heating in open flasks because thioglycollate is volatile.
Old medium should be freshly steamed and the charcoal resuspended before use.
Keep medium cool during transport but do not freeze.

주문정보

Cat No. 품명 단위 규격 CAS No. 수량 비고
CM0425J Amies Transport Medium BT 250g