제품 상세
Bacteroides spp. 등 배양이 까다로운 혐기성 균 및 기타 혐기성 균들을 배양하기 위한 영양배지.
N-S Anaerobe Selective Supplement (SR0107), G-N Anaerobe Selective Supplement (SR0108), Neomycin Suppelement (SR0163) 등의 선택 첨가제들을 첨가하여 선택적으로 만들 수 있다.
Typical Formula* | gm/litre |
Peptone | 16.0 |
Yeast extract | 7.0 |
Sodium chloride | 5.0 |
Starch | 1.0 |
Dextrose | 1.0 |
Sodium pyruvate | 1.0 |
Arginine | 1.0 |
Sodium succinate | 0.5 |
L-cysteine HCl | 0.25 |
Sodium bicarbonate | 0.4 |
Ferric pyrophosphate | 0.5 |
Haemin | 0.005 |
Vitamin K | 0.0005 |
Dithiothreitol | 0.25 |
Agar | 12.0 |
pH 6.8 ± 0.2 @ 25°C |
|
* Adjusted as required to meet performance standards
Directions
Suspend 46 g in 1 litre of distilled water. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50-55°C and aseptically add 5-10% sterile Defibrinated Horse Blood SR0050.
Mix well and pour into sterile Petri dishes.
Appearance
Dehydrated medium: Straw coloured, free-flowing powder
Prepared medium: Straw coloured gel
Quality control
Positive controls: | Expected results |
Peptostreptococcus anaerobius ATCC® 27337 * | Good growth; grey coloured colonies |
Prevotella melaninogenica ATCC® 25845 * | Good growth; grey coloured colonies |
Clostridium perfringens ATCC® 13124 | Good growth; grey coloured colonies |
Negative control: |
|
Uninoculated medium | No change |
* This organism is available as a Culti-Loop®