제품 상세
혐기성 균을 배양하기 위한 액체 배지
Typical Formula* | gm/litre |
Peptone | 16.0 |
Yeast extract | 7.0 |
Sodium chloride | 5.0 |
Starch | 1.0 |
Dextrose | 1.0 |
Sodium pyruvate | 1.0 |
Arginine | 1.0 |
Sodium succinate | 0.5 |
L-cysteine HCl | 0.5 |
Sodium bicarbonate | 0.4 |
Ferric pyrophosphate | 0.5 |
Haemin | 0.005 |
Vitamin K | 0.0005 |
Sodium thioglycollate | 0.5 |
Dithiothreitol | 1.0 |
pH 6.8 ± 0.2 @ 25°C |
|
* Adjusted as required to meet performance standards
Directions
Suspend 35.4 g of Anaerobe Basal Broth in 1 litre of distilled water. Bring to the boil to dissolve. Distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.
Appearance
Dehydrated medium: Straw coloured, free-flowing powder
Prepared medium: Straw/green coloured solution
Quality control
Positive controls: | Expected results |
Peptostreptococcus anaerobius ATCC® 27337* | Turbid growth |
Prevotella loescheii ATCC 15930 | Turbid growth |
Negative control: |
|
Uninoculated medium | No change |
* This organism is available as a Culti-Loop®