제품 이미지
Azide Blood Agar Base 제품소개 : 미생물배양용 분말배지, 혈액 별도 첨가  브 랜 드  : Oxoid

제품 상세

분변, 폐수 및 기타 검체에 존재하는 streptococci와 staphylococci를 검출 및 분리하기 위한 선택 배지.
혈액을 첨가하여 용혈 반응의 종류를 결정하는데 사용할 수도 있다.


Typical Formula*

gm/litre

Tryptose

10.0

`Lab-Lemco’ powder

3.0

Sodium chloride

5.0

Sodium azide

0.2

Agar

12.0

pH 7.2 ± 0.2 @ 25°C

 


* Adjusted as required to meet performance standards


Directions

Suspend 30 g in 1 litre of distilled water and bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. For azide blood agar, cool to 45-50°C and add 5% of sterile blood.


Appearance
Dehydrated medium: Straw coloured, free-flowing powder
Prepared medium: Straw coloured gel


Quality control

Positive controls:

Expected results

Enterococcus faecalis ATCC® 29212 *

Good growth; white/grey colonies

Staphylococcus aureus ATCC® 25923 *     

Good growth; white colonies

Negative controls:

 

Proteus hauseri ATCC® 13315 *

Inhibited

Escherichia coli ATCC® 25922 *

Inhibited


* This organism is available as a Culti-Loop®


Precautions

Proteus and Escherichia species may not always be inhibited on the Edward’s formulation.
Always use a light inoculum for best selective results.
Anaerobic incubation will enhance haemolytic reactions.
Haemolytic reactions will not be typical on Packer’s modification of Azide Blood Agar Base. Streptococcus lactis will not grow on Packer’s modification with 5% sheep blood.
Read the section on Hazard Precautions for azide-containing media.

주문정보

Cat No. 품명 단위 규격 CAS No. 수량 비고
CM0259B Azide Blood Agar Base BT 500g