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제목 [ThermoFisher Scientific]iBlot 3 Western Blot Transfer System

2023-03-03
분야 생명과학 브랜드 Thermo Scientific
첨부파일 iBlot 3 Flyer_국문_2023(공유용).pdf (2.47 MB)
내용 트렌스퍼를 쿨~하게!

Invitrogen™ iBlot™ 3 Western Blot Transfer System은 미리 준비된 소모품으로 최고의 퍼포먼스와 편리함을 자랑하는 차세대 단백질 트랜스퍼 시스템입니다. 업그레이드된 장비는 실험실 생산을 높이고 일관되고 재현성있는 결과를 나타냅니다. 다음의 기능을 경험하세요.
  • 생산성의 최대화: 독립적으로 조작할 수 있는 두 개의 트랜스퍼 스테이션으로 동시에 4개의 mini gel 또는 2개의 midi gel을 다른 프로그램으로 작동
  • 더 나아진 트랜스퍼 효율: wet tank 트랜스퍼 또는 다른 빠른 트랜스퍼 방법보다 더 나은 단백질 트랜스퍼 효율 기대
  • 일관되고 재현성있는 결과: 새 냉각 기능으로 열 발생 감소 및 트랜스퍼 재현성을 개선

● 1분 동영상 보기

The next generation of performance and convenience
The iBlot 3 Western Blot Transfer System combines an innovative, all-new robust design that delivers exceptional performance and convenience with proven iBlot technology. The iBlot 3 System is a true self-contained, plug-and-play system, with an integrated power supply and pre-packaged ready-to-use transfer stacks. Setup, teardown, and cleanup only takes minutes compared with the much more cumbersome and hands-on steps required for wet-tank transfer.


Maximum Productivity
Now you can perform reliable, high-quality transfers faster than ever—in as few as 3 minutes. You can also save time that would be spent waiting for transfer device availability. Two independently controlled transfer stations allow you to share the device with your coworker while simultaneously running four mini gels or two midi gels with two different programs. In addition, you can reduce method setup time by selecting preprogrammed methods from the user-friendly touchscreen interface. Programs can also be customized to fit your needs.

Better Transfer Efficiency
With the iBlot 3, you can achieve as good or better protein transfer efficiency compared with wet tank transfer and other rapid transfer methods.



The iBlot 3 system demonstrates better transfer efficiency. (A) Hsp70: Invitrogen NuPAGE 4–12% Bis-Tris mini gels were loaded with 20, 15, 10, 5, 2.5, 1.25, 0.625, and 0.312 μg of A431 lysate per lane, respectively. After gel electrophoresis, the proteins were transferred using the method and conditions shown above. Immunoprocessing was completed using the Invitrogen Bandmate Automated Western Blot Processor. Blots were incubated with Thermo Scientific Pierce Clear Milk Blocking Buffer for 30 minutes. Hsp70 primary antibody (1:1,000 in clear milk) was added to blots and incubated for 12 hours at room temperature. Primary antibody was removed and GAM-HRP secondary antibody (1:120,000 in TBST) was added to blots and incubated for 2 hours. Thermo Scientific SuperSignal West Dura chemiluminescent substrate was used for detection. Invitrogen iBright FL1500 Imaging System was used for image capture. Local background corrected volume per lysate was plotted for each lane. (B) 4EBP1: Invitrogen Novex 16% Tricine mini gels were loaded with 10, 5, 2.5, 1.25, 0.625, 0.312, 0.156, and 0.078 μg of A431 lysate per lane, respectively. After gel electrophoresis, the proteins were transferred using the method and conditions shown above. Immunoprocessing was completed using the Bandmate Automated Western Blot Processor. Blots were incubated with Pierce Clear Milk Blocking Buffer for 30 minutes. 4EBP1 primary antibody (1:1,000 in clear milk) was added to blots and incubated for 12 hours at room temperature. Primary antibody was removed and GAR-HRP secondary antibody (1:175,000 in TBST) was added to blots and incubated for 2 hours. SuperSignal West Dura chemiluminescent substrate was used for detection. The iBright FL1500 instrument was used for image capture. Local background corrected volume per lysate was plotted for each lane. Note: GAM = goat anti-mouse, HRP = horse radish peroxidase, TBST = tris-buffered saline with Tween™ (solution), GAR = goat anti-rabbit.